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Apath LLC huh-7.5 cells
Huh 7.5 Cells, supplied by Apath LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh-7%2E5+cells/huh7+5+cells/pmc11578187-244-25-30
Average 90 stars, based on 1 article reviews
huh-7.5 cells - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

CRISPR:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Construct:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Expressing:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Immunoprecipitation:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

In Vivo:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Infection:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Virus:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Lysis:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Protease Inhibitor:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Modification:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Polymerase Chain Reaction:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Functional Assay:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Sequencing:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Mutagenesis:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Derivative Assay:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Binding Assay:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Isolation:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Knock-Out:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Inhibition:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Transduction:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Serial Dilution:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Flow Cytometry:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Cytometry:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Incubation:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Concentration Assay:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Luciferase:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Selection:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.

Cell Culture:

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Human hepatoma-derived Huh-7.5 cells (55) and H1-HeLa were obtained from Apath LLC and the American Type Culture Collection, respectively, maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 3 to 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and tested negative for mycoplasma by polymerase chain reaction (PCR) assay (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

Article Title: The risk of COVID-19 death is much greater and age dependent with type I IFN autoantibodies
Article Snippet: Huh-7.5 cells are available on request from C.M.R. under an MTA with The Rockefeller University and Apath LLC.

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Floxed Pdap1 f mice were provided by M. Di Virgilio of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany, and Huh-7.5 cells were provided by Apath LLC, under the terms of a material transfer agreement.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication
Article Snippet: Cells and viruses 293T and human liver-derived Huh-7.5 cells were obtained from ATCC and Apath LLC, respectively.

Article Title: Hepatoviruses promote very-long-chain fatty acid and sphingolipid synthesis for viral RNA replication and quasi-enveloped virus release.
Article Snippet: Huh-7.5 cells used in this study were provided by Apath LLC under a material transfer agreement (roth@apath.com).

Article Title: Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses
Article Snippet: Human hepatoma-derived Huh-7.5 cells were obtained from Apath LLC.

Article Title: Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.
Article Snippet: Relatively little is known of the mechanisms underlying hepatitis A virus (HAV) genome replication.. Unlike other well-studied picornaviruses, HAV RNA replication requires the zinc finger protein ZCCHC14 and non-canonical TENT4 poly(A) polymerases with which it forms a complex.. The ZCCHC14–TENT4 complex binds to a stem-loop located within the internal ribosome entry site (IRES) in the 5’ untranslated RNA (5’UTR) and is essential for viral RNA synthesis, but the underlying mechanism is unknown.

Article Title: An entropic safety catch controls hepatitis C virus entry and antibody resistance
Article Snippet: Huh-7.5 cells were acquired from Apath LLC.



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